Modified, Rapid, and Accurate Method for the Quantification of Mucopolysaccharides in Urine

A modified colorimetric method for the determination of urinary mucopolysaccharides is presented. It involves reaction with Alcian blue dye under relatively mild conditions and reading in the visible spectrophotometry at 608 nm. It is more sensitive than the original method as it measures all classes of urinary mucopolysaccharides. Results in normal children at different ages corrected to creatinine excretion are reported. *Corresponding author: Zouhair Habbal M, Department of Pathology and Laboratory Medicine, School of Medicine, American University of Beirut Medical Center. P.O. Box 11-0236 Riad El-Solh, Beirut 1107 2020 Lebanon, Tel: +961-1350000/5220; Fax: +961-1-370845; E-mail: [email protected] Received May 11, 2014; Accepted October 18, 2014; Published October 21, 2014 Citation: Habbal MZ, Cherry MA (2014) Modified, Rapid, and Accurate Method for the Quantification of Mucopolysaccharides in Urine. J Glycobiol 3: 111. doi:10.4172/2168-958X.1000111 Copyright: © 2014 Habbal MZ, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.